Biomass Breakdown Technology



Articles and Patents (download PDFs from ResearchGate)

1. Muller M, Prade RA, Segato F, Atiyeh HK, Wilkins MR. Continuous xylanase production with Aspergillus nidulans under pyridoxine limitation using a trickle bed reactor. Bioresource technology. 2015;188:219-25.


3. Mandelli F, Oliveira Ramires B, Couger MB, Paixao DA, Camilo CM, Polikarpov I, et al. Draft Genome Sequence of the Thermophile Thermus filiformis ATCC 43280, Producer of Carotenoid-(Di)glucoside-Branched Fatty Acid (Di)esters and Source of Hyperthermostable Enzymes of Biotechnological Interest. Genome announcements. 2015;3(3).

4. Cota J, Correa TL, Damasio AR, Diogo JA, Hoffmam ZB, Garcia W, et al. Comparative analysis of three hyperthermophilic GH1 and GH3 family members with industrial potential. N Biotechnol. 2015;32(1):13-20.

5. Segato F, Damasio AR, de Lucas RC, Squina FM, Prade RA. Genomics review of holocellulose deconstruction by aspergilli. Microbiol Mol Biol Rev. 2014;78(4):588-613.

6. Santos CR, Polo CC, Costa MC, Nascimento AF, Meza AN, Cota J, et al. Mechanistic strategies for catalysis adopted by evolutionary distinct family 43 arabinanases. The Journal of biological chemistry. 2014;289(11):7362-73.

7. Ribeiro LFC, De Lucas RC, Vitcosque GL, Ribeiro LF, Ward RJ, Rubio MV, et al. A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology. Biotechnology for biofuels. 2014;7(1):115.

8. Prade RA, Wang H, inventors; The Board of Regents for Oklahoma State University (203 Whitehurst, Oklahoma State University, Stillwater OK, 74078, US), assignee. THERMOCELLULASES FOR LIGNOCELLULOSIC DEGRADATION. United States patent 8,847,031 B2. 2014.

9. Muller M, Segato F, Prade RA, Wilkins MR. High-yield recombinant xylanase production by Aspergillus nidulans under pyridoxine limitation. Journal of industrial microbiology & biotechnology. 2014;41(10):1563-70.

10. Youssef NH, Couger MB, Struchtemeyer CG, Liggenstoffer AS, Prade RA, Najar FZ, et al. The genome of the anaerobic fungus Orpinomyces sp. strain C1A reveals the unique evolutionary history of a remarkable plant biomass degrader. Applied and environmental microbiology. 2013;79(15):4620-34.

11. Segato F, Damasio AR, Goncalves TA, Murakami MT, Squina FM, Polizeli M, et al. Two structurally discrete GH7-cellobiohydrolases compete for the same cellulosic substrate fiber. Biotechnology for biofuels. 2012;5:21.

12. Segato F, Damasio AR, Goncalves TA, de Lucas RC, Squina FM, Decker SR, et al. High-yield secretion of multiple client proteins in Aspergillus. Enzyme and microbial technology. 2012;51(2):100-6.

13. Saykhedkar S, Ray A, Ayoubi-Canaan P, Hartson SD, Prade R, Mort AJ. A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover. Biotechnology for biofuels. 2012;5(1):52.

14. Ray A, Saykhedkar S, Ayoubi-Canaan P, Hartson SD, Prade R, Mort AJ. Phanerochaete chrysosporium produces a diverse array of extracellular enzymes when grown on sorghum. Applied microbiology and biotechnology. 2012;93(5):2075-89.

15. Goncalves TA, Damasio AR, Segato F, Alvarez TM, Bragatto J, Brenelli LB, et al. Functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides. Bioresource technology. 2012;119:293-9.

16. dos Santos CR, Paiva JH, Meza AN, Cota J, Alvarez TM, Ruller R, et al. Molecular insights into substrate specificity and thermal stability of a bacterial GH5-CBM27 endo-1,4-beta-D-mannanase. Journal of structural biology. 2012;177(2):469-76.

17. Damásio ARdL, Pessela BC, Segato F, Prade RA, Guisan JM, Polizeli MdLTM. Improvement of fungal arabinofuranosidase thermal stability by reversible immobilization. Process Biochemistry. 2012;47(12):2411-7.

18. Damásio ARdL, Pessela BC, Mateo C, Segato F, Prade RA, Guisan JM, et al. Immobilization of a recombinant endo-1,5-arabinanase secreted by Aspergillus nidulans strain A773. Journal of Molecular Catalysis B: Enzymatic. 2012;77(0):39-45.

19. Damasio AR, Ribeiro LF, Ribeiro LF, Furtado GP, Segato F, Almeida FB, et al. Functional characterization and oligomerization of a recombinant xyloglucan-specific endo-beta-1,4-glucanase (GH12) from Aspergillus niveus. Biochimica et biophysica acta. 2012;1824(3):461-7.

20. Blaylock MJ. Energy Crop Patent Issued to Edenspace: Technology Helps to Produce Sugars from Cellulosic Biomass. Edensapce News Release. 2012.

21. Wang H, Squina F, Segato F, Mort A, Lee D, Pappan K, et al. High-temperature enzymatic breakdown of cellulose. Applied and environmental microbiology. 2011;77(15):5199-206.

22. Souza TA, Santos CR, Souza AR, Oldiges DP, Ruller R, Prade RA, et al. Structure of a novel thermostable GH51 alpha-L-arabinofuranosidase from Thermotoga petrophila RKU-1. Protein science : a publication of the Protein Society. 2011;20(9):1632-7.

23. dos Santos CR, Squina FM, Navarro AM, Oldiges DP, Leme AF, Ruller R, et al. Functional and biophysical characterization of a hyperthermostable GH51 alpha-L-arabinofuranosidase from Thermotoga petrophila. Biotechnology letters. 2011;33(1):131-7.

24. Damásio ARdL, Silva TM, Almeida FBdR, Squina FM, Ribeiro DA, Leme AFP, et al. Heterologous expression of an Aspergillus niveus xylanase GH11 in Aspergillus nidulans and its characterization and application. Process Biochemistry. 2011;46(6):1236-42.

25. Cota J, Alvarez TM, Citadini AP, Santos CR, de Oliveira Neto M, Oliveira RR, et al. Mode of operation and low-resolution structure of a multi-domain and hyperthermophilic endo-beta-1,3-glucanase from Thermotoga petrophila. Biochemical and biophysical research communications. 2011;406(4):590-4.

26. Squina FM, Santos CR, Ribeiro DA, Cota J, de Oliveira RR, Ruller R, et al. Substrate cleavage pattern, biophysical characterization and low-resolution structure of a novel hyperthermostable arabinanase from Thermotoga petrophila. Biochemical and biophysical research communications. 2010;399(4):505-11.

27. Santos CR, Squina FM, Navarro AM, Ruller R, Prade R, Murakami MT. Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of the catalytic domain of a hyperthermostable endo-1,4-beta-D-mannanase from Thermotoga petrophila RKU-1. Acta crystallographica Section F, Structural biology and crystallization communications. 2010;66(Pt 9):1078-81.

28. Santos CR, Meza AN, Hoffmam ZB, Silva JC, Alvarez TM, Ruller R, et al. Thermal-induced conformational changes in the product release area drive the enzymatic activity of xylanases 10B: Crystal structure, conformational stability and functional characterization of the xylanase 10B from Thermotoga petrophila RKU-1. Biochemical and biophysical research communications. 2010;403(2):214-9.

29. Prade RA, Squina FM, inventors; The Board of Regents for Oklahoma State University (203 Whitehurst, Oklahoma State University, Stillwater OK, 74078, US), assignee. Thermohemicellulases for Lignocellulosic Degradation2010.

30. Squina FM, Prade RA, Wang H, Murakami MT. Expression, purification, crystallization and preliminary crystallographic analysis of an endo-1,5-alpha-L-arabinanase from hyperthermophilic Thermotoga petrophila. Acta crystallographica Section F, Structural biology and crystallization communications. 2009;65(Pt 9):902-5.

31. Squina FM, Mort AJ, Decker SR, Prade RA. Xylan decomposition by Aspergillus clavatus endo-xylanase. Protein expression and purification. 2009;68(1):65-71.

32. Mort A, Gao S, Wu X, Prade R. The need for pure enzymes that degradecell wall polysaccharides and how to get them. Plant Biosystems. 2005;139:84-7.

33. Fu J, Prade R, Mort A. Expression and action pattern of Botryotinia fuckeliana (Botrytis cinerea) rhamnogalacturonan hydrolase in Pichia pastoris. Carbohydrate research. 2001;330(1):73-81.

34. Prade RA, Zhan D, Ayoubi P, Mort AJ. Pectins, pectinases and plant-microbe interactions. Biotechnology & genetic engineering reviews. 1999;16:361-91.

35. Prade RA. Xylanases: from biology to biotechnology. Biotechnology & genetic engineering reviews. 1996;13:101-31.

36. Milagres A, Prade R. Production of xylanases from Penicillium janthinellum and its use in the recovery of cellulosic textile fibers. Enzyme and microbial technology. 1994;16:627-32.

37. Milagres A, Lacis L, Prade R. Characterization of xylanase production by a local isolate of Penicillium janthinellum. Enzyme and microbial technology. 1993;15:248-53.